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The concentration of acetylcholine (a neurotransmitter) in a sample can be determined from the pH changes that accompany its hydrolysis. When the sample is incubated with the enzyme acetylcholinesterase, acetylcholine is converted to choline and acetic acid, which dissociates to yield acetate and a hydrogen ion: In a typical analysis, 15 mL of an aqueous solution containing an unknown amount of acetylcholine had a pH of 7.65 . When incubated with acetylcholinesterase, the pH of the solution decr
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