Electroporation buffer and protoplast culture medium should be sterilized by filtration through a 0.2 μm bottle-top filter in a laminar flow hood because of the presence of sucrose in the media.

International Journal of Plant Sciences, Vol. 158, No. 5 (Sep., 1997), pp. 537-542 (6 pages) Experiments to maximize the isolation and purification of viable protoplasts from shoot cultures of ...

A protoplast-release-inducing protein (PR—IP) which is released from mating-type plus (mt+) cells and induces the release of gametic protoplasts from mating-type minus (mt-) cells of Closterium was ...

Research probing the secret of how some single plant cells can regenerate into whole plants will benefit from a new culture protocol developed by RIKEN scientists 1. Some plants naturally possess the ...