Figure 5: H3.3 localizes to pericentromeric domains surrounding NLBs in the male pronucleus only. (a, b) Zygotes injected with mRNA for H3.3–GFP WT were analysed under confocal microscopy (n = 73).

To create transgenic mice, the most common method is pronuclear microinjection, where a DNA construct containing the desired gene is injected directly into the pronucleus of a fertilized mouse egg ...

FemtoJet ® microinjector for pronucleus injection ES cell transfer capillaries, e.g. Eppendorf Piezo Drill Tips ES, TransferTips ES injection capillaries from BioMedical Instruments ...