First, the cells are incubated with the cryoprotectant at 37 °C (98.6 °F) – human body temperature – for a few hours, before being frozen. Later, the samples were thawed and examined with ...

The key to PVA’s cryoprotectant properties is its size, Gibson’s group has found (Biomacromolecules 2013, DOI: 10.1021/bm400217j).

ICSI showed fertility of induced spermatozoa only in vitrification using DMSO free cryoprotectant group. Conclusions: An optimal cryopreservation protocol enhances the chances for successful fertility ...

Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing. Proceedings of the National Academy of Sciences, 2019; 116 (16): 7738 DOI: 10.1073/pnas.1808645116 ...

To deliver cryoprotectant into all the nooks and crannies of a larger body from the outside, you generally need to drain the blood out and pump the new solution in through the circulatory system.

However, the mechanism by which COOH-PLL acts as a cryoprotectant remained poorly understood, and this hindered efforts at developing better polyampholyte cryoprotectants.

Cell tissue must not freeze! Polyproline, as a minimal antifreeze protein mimetic, protects cell monolayers from freeze damage Date: December 7, 2017 Source: Wiley Summary: Nature has evolved ...

Based on the sensitivity to cryoprotectant exposure, heart beating stage embryos of E. sinensis are an appropriate stage for further cryopreservation. La réponse des embryons du crabe chinois ...

Freeze tolerance and cryoprotectant synthesis was examined in Hyla regilla, collected from Northern California in the spring and fall. Specimens frozen at 2 C for six and 12 hours had a survival rate ...