A cDNA library derived from antigen positive cells was constructed using the pKE-1 vector. E. coli XL1-blue bacteria are transformed by electroporation of the recombinant plasmids.

About this article Cite this article Porkka, K., Visakorpi, T. Detection of a new, prostate-specific gene by using suppression subtractive hybridization and cDNA library arrays.

If your lab can afford to spend roughly $15,000 (£7,200, academic price) for the RIKEN Mouse Genome Encyclopedia DNABook, maybe you can. The loose-leaf volume contains 60,770 cDNA mouse clones spotted ...

Complementary DNA (cDNA) libraries are standard tools for gene expression studies. In theory, the library contains full-length DNA copies of every mRNA in the starting sample, in abundances ...

A. Tagment cDNA – 6.35 µL total reaction volume Start with 1.25 µL of input DNA in wells of a 384-well microplate Aspirate TD buffer into a 200 µL pipette tip* and place on LV chip on MANTIS.

How do you prepare an RNA sequencing library? This tends to be specific to the platform that is being used for sequencing, and many companies will offer a library preparation kit. You will need to ...