A. Tagment cDNA – 6.35 µL total reaction volume Start with 1.25 µL of input DNA in wells of a 384-well microplate Aspirate TD buffer into a 200 µL pipette tip* and place on LV chip on MANTIS.

How do you prepare an RNA sequencing library? This tends to be specific to the platform that is being used for sequencing, and many companies will offer a library preparation kit. You will need to ...

They constructed a self-epitope expressed library (SEL) using cDNAs from normal mouse prostate and compared its effectiveness against mouse prostate tumors with that of the human cDNA-based ASEL ...

Complementary DNA (cDNA) libraries are standard tools for gene expression studies. In theory, the library contains full-length DNA copies of every mRNA in the starting sample, in abundances ...

Ji Hyung Jun, Cheol Soo Kim, Dae Shik Cho, June Myoung Kwak, Chan Man Ha, Yu Shin Park, Baik Ho Cho, David A. Patton, Hong Gil Nam, Random antisense cDNA mutagenesis as an efficient functional genomic ...

Jian Guo Dong, Woo Taek Kim, Wing Kin Yip, Gregory A. Thompson, Liming Li, Alan B. Bennett, Shang Fa Yang, Cloning of a cDNA encoding 1-aminocyclopropane-1-carboxylate synthase and expression of its ...

We isolated mRNA from Blumeria graminis and prepared a cDNA library using a modification of Epicentre's ExactSTART™ Full-Length cDNA Library Cloning kit. Analysis of the 5′ ends of the cDNA by ...

If your lab can afford to spend roughly $15,000 (£7,200, academic price) for the RIKEN Mouse Genome Encyclopedia DNABook, maybe you can. The loose-leaf volume contains 60,770 cDNA mouse clones spotted ...