At the ESCRS winter meeting, Dimitrios Tsouris, MD, of Greece presented an unexpected case of trypan blue migration in the vitreous cavity during phacoemulsification in a 30-year-old man with ...

Trypan blue is widely used in cell culture labs for selectively staining dead cells and tissues, but some sample types present challenges for accurately assessing cell counts and viability. For these ...

Trypan blue is only effective for quantifying cell viability during late-stage apoptosis, as this is when the membrane physically degrades, enabling the dye to penetrate and stain the non-viable cell.

Trypan Blue Optional: filter the Trypan Blue solution through a 0.2 µm filter to eliminate any aggregates or crystals that may form. Add Trypan Blue to the cell suspension in a 1:1 ratio and mix.

Trypan blue techniques need hands-on work by technicians, can’t be easily automated, and are invasive because cells need to be removed from the bioreactor and stained, he explains.