PLFA 分析虽能区分真菌和细菌的生物量，却因各实验室分析步骤和结果解释的差异，导致不同研究间结果难以比较。 近年来新兴的基于 DNA 测序技术的方法，如定量 PCR（qPCR）和微滴数字 PCR（ddPCR），虽有潜力，但也受到 DNA 提取方法、引物设计等多种因素影响，且 ddPCR 的应用尚未普及。

编辑推荐： 推荐：研究人员针对除草剂离子液体 (ILs)的环境归趋和生态风险展开研究，通过28天土壤降解实验和PLFA分析，发现胆碱基阳离子不影响除草剂阴离子 (2,4-D/MCPA)的降解效率及土壤微生物多样性，但吗啉衍生物阳离子 [BDMorph]+表现出剂量依赖性抗雌激素活性，为新型农用化学品设计提供重要 ...

Bray–Curtis similarity matrices of the PLFA profiles (% of total PLFA) were square-root transformed to even out the contribution of very rare and very dominant PLFAs, and analysed by cluster ...

PLFA are markers for living bacteria and can be used to profile community structure. The ubiquitous presence of microbes requires extreme caution during collection, storage and processing of samples.

PLFA patterns of the SMC shifted because of clear-cutting, but seasonal temporal changes had greater shifts than differences among stand age. The microbial biomass (total PLFA) and bacterial, fungal, ...

Tianli Bao, Liqian Gao, Shanshan Wang, Xueqin Yang, Wei Ren, Yunge Zhao, Moderate disturbance increases the PLFA diversity and biomass of the microbial community in biocrusts in the Loess Plateau ...