1.4 PCR-SSP 严格按照人类红细胞ABO血型-B亚型基因分型试剂盒 (PCR-SSP法)说明书操作。 1.5 ABO基因第6、7外显子直接测序 ...

为解决亚洲人群中RhD阴性个体中亚洲型DEL (RHD*01EL.01)变异检测的难题，韩国世基因医学基金会研究人员开发了一种新型多重实时PCR (mRT-PCR)检测方法。该研究采用等位基因特异性TaqMan水解探针技术，实现了对c.1227G>A突变的快速精准检测。经315例临床样本验证显示100%的敏感性和特异性，检测时间仅需100 ...

PCR-SSP typing for HLA-AB confirmed split antigens of the A9, A10, A19, B5, B12, B15, B16, B17, B21, B22, B40 serotypes in all donors (n =81) where serological splits had been assigned.

HLA-A and B typing was performed either by serology or at the DNA level. HLA-DRB1 alleles were typed using PCR-SSO or PCR-SSP. All the tests were performed in the HLA laboratory of our facility.

During 2014, Allenex (STO:ALNX) has started the development of brand new HLA typing tests based on the method of real-time PCR. The aim is to launch the products during the second quarter of 2015 ...