为解决遗传性血色素沉着症（HH）在植入前遗传学检测（PGT）中依赖间接单倍型分析的局限性，研究人员开发了一种基于聚合酶链反应-限制性片段长度多态性（PCR-RFLP）的直接检测方法。该研究成功验证了针对HFE基因C282Y变体的检测体系，在80例细胞系、38例患者样本和81例胚胎活检中实现>99%的准确率 ...

编辑推荐： （编辑推荐）本研究通过PCR-RFLP技术分析TNF-α-308G>A（rs1800629）SNP在90例复发性流产（RPL）患者和100例健康对照中的分布，发现AA基因型使RPL风险显著提升3.75倍（p=0.027），A等位基因（OR=2.01, p<0.001）及显性模型（GA+AA, OR=2.06）均显示强关联性，为埃及人群RPL的遗传机制提供了新证据。

The phase 1 (fliC) and phase 2 (fljB) Salmonella flagella genes were analyzed by restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR) to aid in the identification of ...

PCR-RFLP was conducted on an 820-bp amplified product of the ureC gene of H. pylori to determine the genetic heterogeneity of 83 pretreatment and 21 post-treatment isolates. Twelve different ...

AFLP-PCR uses many of the same steps as RFLP, SSR, and RAPD, including restriction enzyme digestion of genomic DNA, PCR, and electrophoretic separation of DNA fragments.

ICMR to commercialise novel primers for PCR-RFLP assay to identify species-specific pathogenic mycobacteriaSenior officials in the ICMR said that there are several salient features to this technology ...

Concurrently, a comprehensive review of Marteilia spp. infections has highlighted advances in diagnostic methodologies, including the development of PCR–RFLP assays and in situ hybridisation ...

Several improved features highlight the upgrade of the Media Cybernetics Gel-Pro Analyzer, of interest to those involved in gel analysis and interpretation. This software quantitatively analyzes PCR, ...