传统基于AvaII和EcoO65I限制性内切酶的RT-PCR/RFLP分型方法，虽被广泛用于日本BCoV监测，但最新研究发现该方法对19株日本流行株（包括3株新分离株）存在分型失效问题。 为解决这一技术瓶颈，日本福岛县和爱媛县的研究团队开展改良研究。

通过150例样本（50例未化疗CRC患者、50例化疗CRC患者和50例健康对照）的队列设计，结合显微镜检查、体外培养和巢式PCR技术检测感染情况。 运用PCR-RFLP和序列标签位点 (STS)分析进行亚型鉴定，并通过SSU rRNA基因测序验证结果。 统计学分析采用SPSS 20.0软件完成。

Nucleic acid analysis, genotyping, PCR, and RFLP methodologies are time-consuming techniques using agarose or acrylamide gel electrophoresis—image capture plus gel image interpretations.

Three PCR-based techniques, RAPD, SSR and ISSR-PCR, as well as RFLP methods, were examined for their ability to generate useful polymorphisms using 13 silkworm varieties.

Polymorphism within the mitochondrial NADH-3,4 dehydrogenase (ND-3/4) and control region (D-Loop) was studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP ...

PCR-RFLP was conducted on an 820-bp amplified product of the ureC gene of H. pylori to determine the genetic heterogeneity of 83 pretreatment and 21 post-treatment isolates. Twelve different ...

Background: To explore the consistency between K-ras mutation in peripheral blood and matched tumor tissues detected by PCR-RFLP and analyze the prognostic effects of K-ras mutation in peripheral ...

PCR-RFLP of gG, TK, and ORFB-TK using restriction endonucleases MspI and FokI, respectively, divided all the isolates into two groups. PCR-RFLP of ICP18.5 and ICP4 using restriction endonuclease ...

ICMR to commercialise novel primers for PCR-RFLP assay to identify species-specific pathogenic mycobacteriaSenior officials in the ICMR said that there are several salient features to this technology ...