结论 研究人员成功建立了一种全新的基于 HPLC-MS/MS 系统的 Cers 全局分析和伪靶向分析方法。 该方法整合了数学模型、多种参考源和 HPLC-MS/MS 分析，与非靶向脂质分析方法相比，具有更宽的线性范围和更高的置信度；与靶向脂质分析技术相比，扩大了 Cers 的数据库。

LC-MS是一种精密仪器，具有较高技术门槛，在全球范围内，领先生产商主要有美国安捷伦（Agilent）、美国赛默飞世尔（Thermo Fisher）、美国沃特世（Waters）、美国布鲁克（Bruker）、美国珀金埃尔默（PerkinElmer）、美国AB SCIEX、日本岛津（Shimadzu）等。

通过LC-MS/MS技术鉴定出100种代谢物，结合分子对接发现6'-丙二酰基芦荟素和8-O-葡萄糖基大黄酚与LasR靶点强结合，首次揭示两种芦荟通过干扰群体感应 (QS)系统发挥抗生物膜、抑制绿脓菌素合成的机制，为开发新型抗毒力药物提供科学依据。

Johns Hopkins University researchers, in response to many high performance liquid chromatography (HPLC) systems having issues ...

In the past few years, high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) has matured to a true alternative to antibody-based immunoassays in routine therapeutic drug ...

Advances in instruments and tools, as well as focused collaborations, improve accuracy and extend the range of applications.

Who is this course for? The Complete HPLC & LC-MS course is for those who have basic knowledge of liquid chromatography and would like to greatly enhance it, learning the practical theory about all ...

Module 11: Applied HPLC & LC-MS Maintenance Module 12: Applied Troubleshooting for HPLC & LC-MS You can choose to attend all the modules for a complete training solution, or alternatively, you can ...

“For these applications, conventional nanospray isn’t reliable enough for routine use and is difficult to implement and maintain,” said Strohmeier. “With the HPLC chip, in combination with Agilent’s ...