This protocol describes the regioselective deprotection of single hydroxyls in peracetylated monosaccharides and disaccharides by enzymatic or chemoenzymatic strategies. The introduction of a one ...

Module 3: Fmoc deprotection. The resin is washed with DMF (six times with 2 ml for 15 s) and swollen in 2 ml DMF; then the temperature of the reaction vessel is adjusted to 25 °C.

By incorporating dimethoxytrityl (DMT), an acid labile 5´ protecting group, nucleotide assembly is accomplished through sequential automated cycles of deprotection, coupling, capping, and oxidation.