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Regulatory T cells were characterised as CD4+FoxP3+; in addition, the expression of CD25, GITR and CTLA-4 among this population was also confirmed (Figure 1A).
In contrast to a decline in Foxp3 expression of CD4+CD25+ T cells in the absence of IL-4, the addition of IL-4 led to sustained Foxp3 expression after 3 days of culture (Figure 6A, upper panel).
Yanyan Qu, Shuguang Jin, Aijun Zhang, Baojun Zhang, Xiaoyun Shi, Junjie Wang, Yong Zhao, Gamma-Ray Resistance of Regulatory CD4⁺CD25⁺Foxp3⁺ T Cells in Mice ...
Thymus-derived regulatory T cells (Tregs) expressing CD4, CD25, and the transcription factor Foxp3 play major roles in preventing autoimmunity. The Treg population is enriched in T cells expressing ...
Their PBMC (peripheral blood mono nuclear cell) were harvested and investigated their character by FACScan (fluorescence-activated cell sorter scan) with fluorescent labeled antibodies (CD3, CD8, CD4, ...
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